Immunoinformatics studies of heat shock proteins 27 and 70: Development of potent therapeutic vaccine constructs against human papillomavirus-related cancers

Heat shock proteins (HSPs) improve cross-presentation of linked tumor antigens, thus they can be exploited in therapeutic vaccine design. Herein, silico analyses different constructs were performed based on human papillomavirus (HPV)-16 E7 protein to Homo sapiens/Mus musculus Hsp27 or Hsp70 multiepitope and whole sequence forms. Then, computational comparison between orientations Hsp/E7 was carried out both Finally, molecular docking the designed signaling (TLRs) endocytic (CD14, LOX-1 SREC-1) receptors. Our data represented high-ranked T-cell epitopes potential B-cell Hsp70. Moreover, showed that had better interaction with all receptors than suggesting likely stronger stimulation innate adaptive immunity. All Hsp27/E7 scores especially SREC-1 Hsp70/E7 orientations. Generally, multiepitope-/whole sequence-based Hsp27-E7 fusion more conservancy immunogenicity other constructs. These non-allergenic, non-toxic stable them as promising candidates against HPV-related cancers.